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Influenza A virus is one of the world's major uncontrolled pathogens, causing seasonal epidemics as well as global pandemics. This was evidenced by the recent emergence and now prevalence of the 2009 swine origin pandemic H1N1 influenza A virus. In this study, quantitative proteomics using stable isotope labelling with amino acids in cell culture was used to investigate the changes in the host cell proteome in cells infected with pandemic H1N1 influenza A virus. The study was conducted in A549 cells that retain properties similar to alveolar cells. Several global pathways were affected, including cell cycle regulation and lipid metabolism, and these could be correlated with recent microarray analyses of cells infected with influenza A virus. Taken together, both quantitative proteomics and transcriptomic approaches can be used to identify potential cellular proteins whose functions in the virus life cycle could be targeted for chemotherapeutic intervention.

Original publication

DOI

10.1002/pmic.201100470

Type

Journal article

Journal

Proteomics

Publication Date

14/05/2012

Volume

12

Pages

1431 - 1436

Addresses

Centre for Emergency Preparedness and Response, Health Protection Agency, Porton Down, Salisbury, UK.

Keywords

Lung, Cell Line, Tumor, Epithelial Cells, Humans, Proteome, Blotting, Western, Reproducibility of Results, Isotope Labeling, Proteomics, Influenza, Human, Influenza A Virus, H1N1 Subtype, Host-Pathogen Interactions