FLASH-TB: an Application of Next-Generation CRISPR to Detect Drug Resistant Tuberculosis from Direct Sputum
Tram TTB., Ha VTN., Trieu LPT., Ashton PM., Crawford ED., Thu DDA., Quang NL., Thwaites GE., Walker TM., Anscombe C., Thuong NTT.
Offering patients with tuberculosis (TB) an optimal and timely treatment regimen depends on the rapid detection of Mycobacterium tuberculosis (Mtb) drug resistance from clinical samples. Finding Low Abundance Sequences by Hybridization (FLASH) is a technique that harnesses the efficiency, specificity, and flexibility of the Cas9 enzyme to enrich targeted sequences.