Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples
Lewandowski K., Xu Y., Pullan ST., Lumley SF., Foster D., Sanderson N., Vaughan A., Morgan M., Bright N., Kavanagh J., Vipond R., Carroll M., Marriott AC., Gooch KE., Andersson M., Jeffery K., Peto TEA., Crook DW., Walker AS., Matthews PC.
Influenza is a major global public health threat as a result of its highly pathogenic variants, large zoonotic reservoir, and pandemic potential. Metagenomic viral sequencing offers the potential for a diagnostic test for influenza virus which also provides insights on transmission, evolution, and drug resistance and simultaneously detects other viruses. We therefore set out to apply the Oxford Nanopore Technologies sequencing method to metagenomic sequencing of respiratory samples.