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The utility of modified vaccinia virus Ankara (MVA) as a vector for eliciting AIDS virus-specific cytotoxic T lymphocytes (CTL) was explored in the simian immunodeficiency virus (SIV)/rhesus monkey model. After two intramuscular immunizations with recombinant MVA-SIVSM gag pol, the monkeys developed a Gag epitope-specific CTL response readily detected in peripheral blood lymphocytes by using a functional killing assay. Moreover, those immunizations also elicited a population of CD8+ T lymphocytes in the peripheral blood that bound a specific major histocompatibility complex class I/peptide tetramer. These Gag epitope-specific CD8+ T lymphocytes also were demonstrated by using both functional and tetramer-binding assays in lymph nodes of the immunized monkeys. These observations suggest that MVA may prove a useful vector for an HIV-1 vaccine. They also suggest that tetramer staining may be a useful technology for monitoring CTL generation in vaccine trials in nonhuman primates and in humans.

Original publication

DOI

10.1073/pnas.95.17.10112

Type

Journal article

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Date

08/1998

Volume

95

Pages

10112 - 10116

Addresses

Harvard Medical School, Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.

Keywords

T-Lymphocytes, Cytotoxic, Animals, Macaca mulatta, Humans, Vaccinia virus, Fusion Proteins, gag-pol, Recombinant Proteins, DNA Primers, AIDS Vaccines, Histocompatibility Antigens Class I, Epitopes, Immunization, Base Sequence, Protein Conformation, Genetic Vectors, Simian immunodeficiency virus