Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Nairobi sheep disease (NSD), caused by the viral agent NSD virus (NSDV), is a haemorrhagic fever disease affecting and inducing high mortality in sheep and goat populations. NSDV belongs to the genus Orthonairovirus of the Nairoviridae family from the order Bunyavirales. Other viruses circulating in livestock such as Crimean-Congo haemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV) are members of the same genus and are reported to share antigenic features. There are very few available materials to study NSDV infection both in vitro and in vivo. In the present work, we characterised two monoclonal antibodies generated in mice that recognise NSDV specifically but not CCHFV or DUGV, along with a potential use to define virus-infected cells, using flow cytometry. We believe this tool can be useful for research, but also NSDV diagnostics, especially through immunological staining.

Original publication

DOI

10.3390/v15091876

Type

Journal article

Journal

Viruses

Publication Date

09/2023

Volume

15

Addresses

The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UK.

Keywords

Animals, Goats, Sheep, Mice, Nairovirus, Hemorrhagic Fever Virus, Crimean-Congo, Nairobi sheep disease virus, Nairobi Sheep Disease, Hemorrhagic Disorders, Nucleoproteins, Antibodies, Monoclonal