Diagnosis of SARS-CoV-2 Infection with LamPORE, a High-Throughput Platform Combining Loop-Mediated Isothermal Amplification and Nanopore Sequencing
Peto L., Rodger G., Carter DP., Osman KL., Yavuz M., Johnson K., Raza M., Parker MD., Wyles MD., Andersson M., Justice A., Vaughan A., Hoosdally S., Stoesser N., Matthews PC., Eyre DW., Peto TEA., Carroll MW., de Silva TI., Crook DW., Evans CM., Pullan ST.
LamPORE is a novel diagnostic platform for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA combining loop-mediated isothermal amplification with Nanopore sequencing, which could potentially be used to analyze thousands of samples per day on a single instrument. We evaluated the performance of LamPORE against reverse transcriptase PCR (RT-PCR) using RNA extracted from spiked respiratory samples and stored nose and throat swabs collected at two UK hospitals.