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ContextMeningococcal infection has a high public profile because of its dramatic presentation, high fatality rate, and propensity to occur in outbreaks and clusters of cases. Use of a diagnostic polymerase chain reaction (PCR) assay could enhance laboratory confirmation of cases and guide the public health response in North America.ObjectiveTo assess the performance of a PCR assay for the diagnosis of meningococcal disease after its implementation in a North American setting and to evaluate sensitivity and specificity of the assay for the detection of prevalent bacterial isolates.DesignLaboratory evaluation of the sensitivity and specificity of a PCR assay for Neisseria meningitidis and observational study of a series of cases comparing molecular diagnosis against the criterion standard of conventional laboratory diagnostic tests.SettingA Canadian province with a population of 4 million people.PatientsChildren and adults presenting with suspected meningococcal disease in British Columbia.Main outcome measuresThe sensitivity and specificity of the PCR assay when compared against standard laboratory methods.ResultsThe PCR assay correctly identified all of 38 Canadian isolates of Neisseria meningitidis and correctly assigned the serogroup to each isolate. None of 57 other gram-positive or gram-negative bacteria or yeasts were detected by the PCR assay. In a clinical evaluation, for diagnosis of meningococcal disease, the PCR assay had a sensitivity and specificity of 91% and 76%, respectively, against conventional methods of diagnosis. Use of the PCR assay increased the laboratory confirmation of clinically suspected cases by 36%. During an outbreak, the PCR assay allowed serogroup determination in 3 of 7 cases, aiding in the public health decision to launch an immunization campaign.ConclusionsThe PCR assay is more sensitive than conventional methods for the diagnosis of meningococcal disease, and enhanced surveillance may help direct the public health response to the changing epidemiology of disease in North America.

Original publication

DOI

10.5858/2002-126-1209-eoadpc

Type

Journal article

Journal

Archives of pathology & laboratory medicine

Publication Date

10/2002

Volume

126

Pages

1209 - 1215

Addresses

Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada. andrew.pollard@paediatrics.ox.ac.uk

Keywords

Humans, Neisseria meningitidis, Meningitis, Meningococcal, DNA, Bacterial, DNA Primers, Microbiological Techniques, Survival Rate, Sensitivity and Specificity, Polymerase Chain Reaction, Disease Outbreaks, Adolescent, Adult, Aged, Child, Child, Preschool, Infant, British Columbia, Female, Male