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Next generation sequencing (NGS) has revolutionized genomics research, making it difficult to overstate its impact on studies of Biology. NGS will immediately allow researchers working in non-mainstream species to obtain complete genomes together with a comprehensive catalogue of variants. In addition, RNA-seq will be a decisive way to annotate genes that cannot be predicted purely by computational or comparative approaches. Future applications include whole genome sequence association studies, as opposed to classical SNP-based association, and implementing this new source of information into breeding programmes. For these purposes, one of the main advantages of sequencing vs. genotyping is the possibility of identifying copy number variants. Currently, experimental design is a topic of utmost interest, and here we discuss some of the options available, including pools and reduced representation libraries. Although bioinformatics is still an important bottleneck, this limitation is only transient and should not deter animal geneticists from embracing these technologies.

Original publication

DOI

10.1111/j.1365-2052.2010.02057.x

Type

Journal

Animal genetics

Publication Date

12/2010

Volume

41

Pages

561 - 569

Addresses

Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. miguel.perez@uab.es

Keywords

Animals, RNA, Sequence Analysis, DNA, Sequence Analysis, RNA, Computational Biology, Genotype, Polymorphism, Single Nucleotide, Genome, Genome-Wide Association Study, Metagenomics, DNA Copy Number Variations, Molecular Sequence Annotation, High-Throughput Nucleotide Sequencing